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, Chenyu Zhang Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute of the Chinese Academy of Agricultural Sciences , Hangzhou 310008 , China Search for other works by this author on: Oxford Academic Haoran Liu Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute of the Chinese Academy of Agricultural Sciences , Hangzhou 310008 , China Search for other works by this author on: Oxford Academic Junya Wang Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute of the Chinese Academy of Agricultural Sciences , Hangzhou 310008 , China Search for other works by this author on: Oxford Academic Yuanyuan Li Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute of the Chinese Academy of Agricultural Sciences , Hangzhou 310008 , China Search for other works by this author on: Oxford Academic Dingding Liu Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute of the Chinese Academy of Agricultural Sciences , Hangzhou 310008 , China Search for other works by this author on: Oxford Academic Yuanyuan Ye Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute of the Chinese Academy of Agricultural Sciences , Hangzhou 310008 , China Search for other works by this author on: Oxford Academic Rong Huang Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute of the Chinese Academy of Agricultural Sciences , Hangzhou 310008 , China Search for other works by this author on: Oxford Academic Sujuan Li State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences , Hangzhou 310021 , China Search for other works by this author on: Oxford Academic Liang Chen Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute of the Chinese Academy of Agricultural Sciences , Hangzhou 310008 , China Search for other works by this author on: Oxford Academic Jiedan Chen Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute of the Chinese Academy of Agricultural Sciences , Hangzhou 310008 , China Correspondence: chenjd@tricaas.com Search for other works by this author on: Oxford Academic
https://orcid.org/0000-0002-7507-3947 , Mingzhe Yao Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute of the Chinese Academy of Agricultural Sciences , Hangzhou 310008 , China Correspondence: yaomz@tricaas.com Search for other works by this author on: Oxford Academic Chunlei Ma Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute of the Chinese Academy of Agricultural Sciences , Hangzhou 310008 , China Correspondence: malei220@tricaas.com Search for other works by this author on: Oxford Academic
Journal of Experimental Botany, Volume 75, Issue 3, 2 February 2024, Pages 935–946, https://doi.org/10.1093/jxb/erad430
Published:
31 October 2023
Article history
Received:
27 July 2023
Editorial decision:
24 October 2023
Accepted:
26 October 2023
Published:
31 October 2023
Corrected and typeset:
18 November 2023
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Chenyu Zhang, Haoran Liu, Junya Wang, Yuanyuan Li, Dingding Liu, Yuanyuan Ye, Rong Huang, Sujuan Li, Liang Chen, Jiedan Chen, Mingzhe Yao, Chunlei Ma, A key mutation in magnesium chelatase I subunit leads to a chlorophyll-deficient mutant of tea (Camellia sinensis), Journal of Experimental Botany, Volume 75, Issue 3, 2 February 2024, Pages 935–946, https://doi.org/10.1093/jxb/erad430
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Abstract
Tea (Camellia sinensis) is a highly important beverage crop renowned for its unique flavour and health benefits. Chlorotic mutants of tea, known worldwide for their umami taste and economic value, have gained global popularity. However, the genetic basis of this chlorosis trait remains unclear. In this study, we identified a major-effect quantitative trait locus (QTL), qChl-3, responsible for the chlorosis trait in tea leaves, linked to a non-synonymous polymorphism (G1199A) in the magnesium chelatase I subunit (CsCHLI). hom*ozygous CsCHLIA plants exhibited an albino phenotype due to defects in magnesium protoporphyrin IX and chlorophylls in the leaves. Biochemical assays revealed that CsCHLI mutations did not affect subcellular localization or interactions with CsCHLIG and CsCHLD. However, combining CsCHLIA with CsCHLIG significantly reduced ATPase activity. RNA-seq analysis tentatively indicated that CsCHLI inhibited photosynthesis and enhanced photoinhibition, which in turn promoted protein degradation and increased the amino acid levels in chlorotic leaves. RT-qPCR and enzyme activity assays confirmed the crucial role of asparagine synthetase and arginase in asparagine and arginine accumulation, with levels increasing over 90-fold in chlorotic leaves. Therefore, this study provides insights into the genetic mechanism underlying tea chlorosis and the relationship between chlorophyll biosynthesis and amino acid metabolism.
Amino acid metabolism, Camellia sinensis, chlorophyll, genetic mapping, magnesium chelatase, protein degradation
© The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com
This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/pages/standard-publication-reuse-rights)
Editor: Karl-Josef Dietz Karl-Josef Dietz Editor Bielefeld University , Germany Search for other works by this author on: Oxford Academic
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